![]() Entry into the nuclear pore complex is controlled by a cytoplasmic exclusion zone containing dynamic GLFG-repeat nucleoporin domains. Hypoxic 3D in vitro culture models reveal distinct resistance processes to TKIs in renal cancer cells. Isolation and characterizations of a novel recombinant scFv antibody against exotoxin A of Pseudomonas aeruginosa. Precision Plus Protein Kaleidoscope Standards are a mixture of ten multicolored recombinant proteins (10250 kD). Lactate regulates autophagy through ROS-mediated activation of ERK1/2/m-TOR/p-70S6K pathway in skeletal muscle. Place this handy selection guide magnet on your refrigerator or lab bench to eliminate the guesswork and make protein standard selection simple. Effect of sulfur-containing agrochemicals on growth, yield, and protein content of soybeans (Glycine max (L.) Merr). For size determination on western blots, depending on the visualization method being used, the protein ladder can be unstained, prestained, or with a Strep -tag sequence. We offer a blue prestained protein standard, as well as a colored prestained protein standard with multi-colored. Review other protein ladders in the unstained and prestained protein ladder guide. Recommended loading: 2 - 3 µl for western blots. Easy to identify: Includes green 25 kDa and red 75kDa reference bands. Ready-to-use: Supplied in a loading buffer for direct loading on gels. Sizes range from 10 to 250 kDa which is ideal for accurate molecular weight determination for a wide range of expressed proteins. Key product features: Broad range: 10-180 kDa. Publishing research using ab116027? Please let us know so that we can cite the reference in this datasheet.Īb116027 has been referenced in 5 publications. New England Biolabs offers a selection of highly pure protein standards. Recommended loading: ~2 - 3 µl for western blots.Easy to identify: Includes green ~25 kDa and red ~75kDa reference bands.Ready-to-use: Supplied in a loading buffer for direct loading on gels.The ladder is supplied in gel loading buffer and is ready to use.ĭo not heat, dilute, add reducing agent before loading. ![]() This prestained protein ladder is designed for monitoring protein separated during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins. These unstained protein standards include three high-intensity reference bands (25, 50, and 75 kD). This product was previously called Prism Ultra Protein Ladder (10 - 180 kDa). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). Prestained Protein Ladder ab116027 is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weights for 10 to 180 kDa. ![]()
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